Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
نویسندگان
چکیده
منابع مشابه
Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler.
BACKGROUND 5382insC BRCA1 frameshift mutation is a common founder mutation for many populations worldwide and a high-risk allele for the development of hereditary breast and/or ovarian cancer. Our goal was to develop a novel, reliable and rapid method for its detection. METHODS We developed an asymmetric real-time PCR method with hybridization probes in the LightCycler. Genotyping was perform...
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ژورنال
عنوان ژورنال: BMC Cancer
سال: 2008
ISSN: 1471-2407
DOI: 10.1186/1471-2407-8-61